Part:BBa_K3332062

J23100-RBS-Lpp-OmpA-GFP-terminator

        This is an anchored proteins onto membranes through Lpp-OmpA and use GFP to comformation. We use BBa_K880005 and GFP to verify Lpp-OmpA's function which can anchor proteins onto membranes.

Biology

        Lpp-OmpA is an anchor protein from E. coli, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display.

        EGFP is green fluorescent protein from jellyfish Aequorea Victoria, which has been widely used as reporter for decades. EGFP is fused at N terminal with Lpp-OmpA so that EGFP may be displayed on the surface of E. coli.

Usage

        Here, we used BBa_K880005 to construct the expression system and obtained the composite part BBa_K3332062. Due to the limited time, we did not get the gene in time. As a result, there is a lack of data about this part. The progress of this part remains in the stage of design.

Fig 1. Gene circuit of Lpp-OmpA-EGFP.

References

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1170